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C1889
Sigma
Collagenase from Clostridium histolyticum
sterile-filtered, release of physiologically active rat hepatocytes tested, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid (CDU = collagen digestion units)
| Synonym: | Clostridiopeptidase A |
| CAS Number: | 9001-12-1 |
| Enzyme Commission (EC) Number: | 3.4.24.3 ( BRENDA | IUBMB ) |
| EC Number: | 232-582-9 |
| MDL number: | MFCD00130830 |
Description
| Analysis Note | Also contains clostripain, nonspecific neutral protease, and tryptic activities. |
| Other Notes | View more information on collagenase and cell dissociation at www.sigma-aldrich.com/enzymeexplorer. |
| Preparation Note | Prepared from Type IV (C5138) |
| Unit Definition | One collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions. One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C. One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT. |
Properties
| sterility | sterile-filtered |
| form | lyophilized powder |
| suitability | release of physiologically active rat hepatocytes tested |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| WGK Germany | 1 |
Related Products
References
| reference | Seglen, P.O. Methods Cell Biol. 13, 29 - 83, (1976) Abstract |
| Merck | Merck 13,2505 |
